Fig. 4
From: ALS-linked misfolded SOD1 species have divergent impacts on mitochondria
Presence of AMF7-63 reactive misfolded SOD1 at the mitochondrial surface coincides with increased mitochondrial volume. Flow cytometric analysis of isolated spinal cord mitochondria from 14 week and early symptomatic SOD1G93A rats labeled with misfolded SOD1 specific antibodies AMF7-63 and B8H10. Analysis includes only events previously gated for MTG staining (MTG+). a Quantification of the geometric mean of FSC of AMF7-63+ (green) B8H10+ (blue) and negative (white) mitochondrial subpopulations relative to total population in 14 week and symptomatic SOD1G93A rats. b Quantification of delta mean fluorescence intensity (ΔMFI) of MTG staining of mitochondrial sub-populations relative to total population. c Quantification of percentage of MitoSOX+ mitochondria from mitochondrial subpopulations relative to total population. Each subpopulation was normalized for MTG staining. d Quantification of ΔMFI of TMRM staining of mitochondrial subpopulations relative to total population and normalized to size/MTG. n = 4–5 animals per time point. Not significant (ns), * P < 0.05, ** P < 0.01, **** P < 0.0001