Fig. 5

Catwalk gait analysis shows improved gait performance after Fasudil treatment in α-Syn^A53T mice. a α-Syn^A53T mice were treated with Fasudil at 10 mg/kg bw and 30 mg/kg bw starting at day 50 (DOB = day of birth). Catwalk gait analysis was performed after 200 days, and after the mouse showed clinical symptoms of the disease by impaired rotarod performance. b Footprints of mice were automatically detected by the Noldus Catwalk XT gait analysis system (RF = right front, LF = left front, RH = right hind, LH = left hind). c Different gait parameters like stand (duration in seconds of contact of a paw with the glass plate), swing (duration in seconds of no contact of a paw with the glass plate), step cycle, stride length, print area, print length and print width were analyzed. d-k Run average speed (d), step sequence regularity index as a fractional measure of inter-paw coordination (e), RF and RH print area (f, i), RF and RH swing speed (g, j), print positions of the right paws (h) and RH stride length (k) were significantly improved after Fasudil treatment as compared to untreated α-Syn^A53T controls. All data given as means ± SEM; n(wt ctrl) = 10, n(A53T ctrl) = 10, n(A53T Fas10) = 10, n(A53T Fas30) = 9; *p < 0.05, **p < 0.01, ***p < 0.001, ^§ p = 0.06, n.s. = not significant; T-Test for comparisons between ctrl groups; ANOVA with Dunnett post-hoc test for multiple group comparisons between α-Syn^A53T groups