Fig. 5

Inclusions detected in patients. Inclusions observed in cases 1 (a, e, f, g , h), 2 (c, d), and 3 (b). a A basophilic inclusion in the pontine nucleus (hematoxylin and eosin stain). b A basophilic inclusion in the inferior olivary nucleus (Klüver–Barrera stain). c, d, e  and f FUS-immunopositive labelling of neuronal cytoplasmic inclusions (NCI) in the hippocampal dentate gyrus (c), inferior olivary nucleus (d,  e) and caudate nucleus (f).g and h NCI in the thoracic spinal cord are immunopositive for TATA-binding protein-associated factor 15 (g) and Ewing sarcoma protein (h). Scale bars: 10 μm (a, b, d, f, and h); 30 μm (c); 15 μm (e)