Table 3 Antibodies used for immunohistochemistry
Antigen | Clone | Species | Isotype | Dilution | Antigen retrieval | Manufacturer |
|---|---|---|---|---|---|---|
PLP | Plpc1 | Mouse | IgG2a | 1:3000 | none | AbD Serotec |
HLA-DR | LN3 | Mouse | IgG2b | 1:1000 | Citrate buffer | eBioscience |
Olig2 | Polyclonal | Rabbit | Â | 1:200 | Citrate buffer | Millipore |
GFAP | 6Â F2 | Mouse | IgG1 | 1:200 | Citrate buffer | Monosan |
HSPB1 | EPR7278 | Rabbit | IgG | 1:500 | Citrate buffer | Abcam |
HSPB5 | JAM01 | Mouse | IgG1 | 1:100 | Citrate buffer | In house |
HSPB6 | EPR14458 | Rabbit | IgG | 1:500 | Citrate buffer | Abcam |
HSPB8 | Polyclonal | Rabbit | Â | 1:500 | Citrate buffer | Abcam |
HSPB11 | polyclonal | Rabbit | Â | 1:50 | Citrate buffer (20Â min) | Novus |