Fig. 2

Glial responses in the absence of functional B and T cells at 8 months. a, b GFAP immunohistological analysis for astrocytes and (c, d) Iba1 immunostaining for microglia reveal significant increases in microgliosis in the amyloid-depositing APP transgenic mice (PSAPP/Rag2 ko and PSAPP) in comparison to the non-transgenic Rag2 ko and WT mice. GFAP immunoreactivity (b) – but not Iba1 immunoreactivity (d) – is significantly reduced in the PSAPP/Rag2 ko mice as compared to the PSAPP mice (representative images of cortical stainings are shown on the left). Scale bar = 300 μm (Insert: Scale bar = 40 μm). *p < 0.05. ***p < 0.001. n = 10–14 per group