Open Access

Erratum to: Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse

  • Christopher M. Henstridge1,
  • Rosemary J. Jackson1,
  • JeeSoo M. Kim1,
  • Abigail G. Herrmann1,
  • Ann K. Wright2,
  • Sarah E. Harris3, 4,
  • Mark E. Bastin3, 5,
  • John M. Starr3, 6,
  • Joanna Wardlaw3, 5,
  • Thomas H. Gillingwater2,
  • Colin Smith3, 5, 8,
  • Chris-Anne McKenzie5,
  • Simon R. Cox3, 7,
  • Ian J. Deary3, 7Email author and
  • Tara L. Spires-Jones1, 8Email author
Contributed equally
Acta Neuropathologica Communications20153:83

DOI: 10.1186/s40478-015-0244-9

Received: 13 October 2015

Accepted: 13 October 2015

Published: 9 December 2015

The original article was published in Acta Neuropathologica Communications 2015 3:53

Erratum

The original version of this article [1] unfortunately contained several mistakes. The presentation of Table 2 and 3 was incorrect, in the HTML and PDF versions of this article. The corrected Tables 2 and 3 are given below.
Table 2

Primary antibody information

Western Blots

ANTIBODY

COMPANY

CODE

Dilution

 

PHF1

Peter Davies

 

1:500

Tau13

Covance

MMS-520R-500

1:2000

GAPDH

Abcam,

Ab8245

1:2000

Beta-actin

Abcam

Ab8226

1:2000

Synaptophysin

Abcam

Ab8049

1:5000

Beta-III-tubulin

Abcam

Ab18207

1:1000

MBP

AbD Serotec

MCA409s

1:500

Histone

Abcam

Ab1791

1:1000

VDAC1/Porin

Abcam

Ab34726

1:500

GluN2B

BD Biosciences

610416

1:500

Synapsin

Millipore

AB1543P

1:20000

Neuropathology

ANTIBODY

COMPANY

CODE

Dilution

Pre-treatment

Beta Amyloid (BA4)

Dako

M087201-2

1:100

98% formic acid 5 min

Alpha Synuclein

Life Technologies

32-8100

1:200

Pressure cooker/formic acid

TDP-43

2B Scientific

CAC-TIP-PTD-MO1

1:4000

Pressure cooker/citric acid

pTau (AT8)

Thermo

MN1020

1:2500

None

Ubiquitin

Dako

Z0458

1:500

Pressure cooker/citric acid

GFAP

Dako

Z0334

1:800

None

CD68

Dako

M0876

1:100

Pressure cooker/citric acid

Array Tomography

ANTIBODY

COMPANY

CODE

Dilution

Secondary Antibody

AW7

Dominic Walsh

 

1:1000

Donkey α Rabbit – AF488

Synaptophysin

Abcam

Ab8049

1:50

Donkey α Mouse – AF594

ApoE

Abcam

Ab7620

1:50

Donkey α Goat – AF647

PSD95

Abcam

Ac12093

1:50

Donkey α Goat – AF488

Table 3

Semi-quantitative scoring of neuropathological markers

 

LBC

AD

 

LBC

AD

Region

Stain

Score

Region

Stain

Score

BA9

TDP43

-

++

BA41/42

TDP43

-

+

pTAU

-

+++

pTAU

-

+++

BA4

+

+++

BA4

+

+++

a-Syn

-

-

a-Syn

-

-

GFAP

+

+++

GFAP

++

+++

CD68

+

+++

CD68

+

+

UBIQ

+

+++

UBIQ

+

+++

BA44/45

TDP43

+

+

EC

TDP43

+

++

pTAU

-

+++

pTAU

+

+++

BA4

-

+++

BA4

++

+++

a-Syn

-

-

a-Syn

-

-

GFAP

+

++

GFAP

+

+++

CD68

++

++

CD68

+

++

UBIQ

+

+++

UBIQ

++

+++

BA46

TDP43

+

++

BA17

TDP43

+

++

pTAU

-

+++

pTAU

-

+++

BA4

-

+++

BA4

+

+++

a-Syn

-

-

a-Syn

-

-

GFAP

+

++

GFAP

+

+++

CD68

+

++

CD68

+

+

UBIQ

+

+++

UBIQ

+

+++

BA6/8

TDP43

+

++

BA24

TDP43

+

++

pTAU

-

+++

pTAU

-

+++

BA4

+

+++

BA4

-

+++

a-Syn

-

-

a-Syn

-

-

GFAP

+

+++

GFAP

+

++

CD68

+

+++

CD68

+

++

UBIQ

+

+++

UBIQ

+

+++

BA9 = Prefrontal cortex, BA44/45 = Broca’s area, BA46 = Dorsolateral Prefrontal cortex, BA6/8 = Premotor cortex, BA41/42 = Superior Temporal cortex, EC = Entorhinal cortex, BA17 = Primary Visual cortex, BA24 = Anterior Cingulate cortex. “-” = no pathology, “+” = mild pathology, “++” = moderate pathology, “+++” = strong pathology. Example images for each score are found in Supplementary Figure 2.

In addition, Fig. 9 was presented incorrectly, in that the label on panel 9D should read “Post-Synapse Volume”. The correct version of Fig. 9 is also provided below.
Fig. 9

Using array tomography to assess the presence of synaptotoxic proteins. Representative images from a single region of interest (crop) captured within the LBC1936 EC (A+C) or the AD EC (B+D). Each image is a single plane from a 3D stack, which has been thresholded/binarised and single-slice objects removed to eliminate background. Sections were stained for synaptophysin, PSD95, and AW7 (A+B) or ApoE (C+D). Synaptically located staining is highlighted with white circles. C. Scale bar = 2μm.

The Conclusions and Authors Contributions sections also contain a number of typing and spacing errors that have been updated. Please see the corrected text provided below.

Lastly, the reference list has been updated to include all named authors up to the first 30.

The original article has been updated to reflect all the above changes.

Notes

Declarations

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Centre for Cognitive and Neural Systems, University of Edinburgh
(2)
Centre for Integrative Physiology, University of Edinburgh
(3)
Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh
(4)
Medical Genetics Section, University of Edinburgh Centre for Genomic and Experimental Medicine and MRC Institute of Genetics and Molecular Medicine, Western General Hospital
(5)
Centre for Clinical Brain Sciences, University of Edinburgh, Chancellor’s Building, 49 Little France Crescent
(6)
Geriatric Medicine Unit, University of Edinburgh, Western General Hospital
(7)
Department of Psychology, CCACE, University of Edinburgh
(8)
Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh

Reference

  1. Henstridge CM, Jackson RJ, Kim JSM, Herrmann AG, Wright AK, Harris SE, Bastin ME, Starr JM, Joanna W, Gillingwater TH, Colin S, Chris-Anne MK, Cox SR, Deary IJ, Spires-Jones TL et al (2015) Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse. Acta Neuropathologica Communications 3:53. doi:10.1186/s40478-015-0232-0 PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Henstridge et al. 2015

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