Figure 1
From: Neuron-to-neuron α-synuclein propagation in vivo is independent of neuronal injury
Assessment of transduction efficiency at the mRNA level. Analyses were performed at 6 weeks post treatment. (a) Rats received a single injection of hα-syn-AAV prep 1 (n = 3) or prep 2 (n = 3) into the vagus nerve. RT-PCR was performed to detect WPRE or, as a control, rat α-syn mRNA in DMnX-containing samples from the left (injected side) MO as well as in samples from the left dorso-medial (dm) and ventro-lateral (vl) pons. Specific bands were detected at 204 (WPRE) and 117 (rat a-syn) bp. (b) Animals received a single injection of vehicle (controls, n = 4), hα-syn-AAV prep 1 (n = 7) or hα-syn-AAV prep 2 (n = 7). Total (rat + human) α-syn mRNA levels were assayed by qRT-PCR in DMnX-containing samples from the left (injected side) MO. Values are means ± SEM. *P <0.05 vs. the control group (one-way ANOVA followed by Tukey’s post hoc test, F2,17 = 5.26).