Figure 6From: Transcript profiling of different types of multiple sclerosis lesions yields FGF1 as a promoter of remyelination FGF1 enhances remyelination in organotypic cerebellar slice cultures. After toxic demyelination, cerebellar slice cultures were allowed to remyelinate for 14 days in the absence or presence of 100 ng/ml FGF1. Myelination was assessed by immunostaining (a, b) and quantitative PCR (c–e). a MBP (red), NFL (green) scale bar = 50 μm b Quantification of MBP+/NFL+ area ratio in FGF1 treated slices compared to untreated controls. MBP+ myelin formation is promoted by FGF1. Students t-test *P = 0.0341. c-e After 14 days RNA was extracted, cDNA obtained and transcript levels of c MBP, d Mag and e Mog were measured by qPCR. One-way ANOVA **P < 0.01; *P < 0.05; All error bars represent SEM from three independent experiments.Back to article page