Figure 5

FGF1 promotes myelination in dissociated spinal cord cultures. Myelinating cultures were treated with different concentrations of FGF1 from 22 DIV to 26 DIV and then stained for myelin (MBP in green) and axons (SMI-31 in red). a FGF1 treated cultures showed enhanced levels of MBP⁺ myelin sheath as compared to the control cultures. Magnification: left panel = 10X, right panel = 40X. b Quantitative evaluation:FG1 promotes myelination ****P <0.0001. Error bars represent SEM of two experiments. Myelinating cultures were treated with 100 ng/ml FGF1 for different time periods. Axonal density calculated as pixel for NFL (different FGF1 dosages/control) were 1.17 for 5 ng/ml FGF1, 1.2 for 50 ng/ml FGF1 and 1.06 for 50 ng/ml FGF1. c 16 days (12 DIV to 28 DIV) and d 6 days (12 DIV to 18 DIV). The myelination was enhanced at day 18 and 24, but unaltered at day 28. ****P <0.0001. Significance of data values was analyzed using T-test. Error bars represent SEM from three independent experiments. The axonal densities (FGF1/control) ranged between 0.98 and 1.02 in the experiments shown in c and d.