Figure 2

Increased numbers and enhanced proliferation of T _eff in T _reg -depleted mice. (A), (D) Representative panoramic pictures of spinal cord cryosections from mice treated with DTx (A) or PBS (D) stained with anti-Foxp3 (green), anti-CD4 (red) and anti-CD11b Ab (grey) (scale bar: 200 μm). (B), (C), (E), (F) Magnified pictures of the indicated regions (A) and (D) (scale bar: 50 μm). (G) Mean absolute numbers (± SEM) of T_eff (CD45⁺CD4⁺Foxp3⁻ cells) isolated from LN and spinal cord of DEREG mice two days after DTx or PBS treatment (n = 11 mice per group, pooled data from three independent experiments). (H) Mean frequency (± SEM) of IFNγ⁺ cells in T_eff isolated from LN and spinal cord of DEREG mice two days after DTx or PBS treatment (n = 5 mice per group, representative data from three independent experiments) (I) EdU was injected i.p. one day after DTx or PBS treatment and 16 hours later lymphocytes were isolated and stained for EdU. Mean frequency (± SEM) of EdU⁺ cells within T_eff (n = 13-14 mice per group, pooled data from four independent experiments). (*P < 0.05, **P < 0.01, t-test).