Figure 6

Correlation of immunolabeled LM and EM images of Pick-like inclusions in two CBD cases. A densely packed round inclusion (Pick-like inclusion) from a CBD case (case 3) labeled with anti-PHF antibody (AT8) visualized with QD 655, also labeled with Alexa 488 for more precise confocal images (A). Correlated LM and EM images (B, C) showed that tau immunoreactivity around the cavity on LM corresponded to bundles of tau filaments that were not arranged as parallely as in AD (C, rectangle d; D). Note that these filaments were intermingled with ribosomes (D, arrow). (E) The ultrastructure of Pick-like inclusions in another case of CBD (case 4) also revealed randomly assembled tau filaments with occasional formation of paired helical filaments (a periodicity of 130 nm, arrow). Scale bars in A to C = 3 μm; D = 50 nm; E = 100 nm. A to D, case 3; E, case 4.