Figure 4

Exposure of cortical somata to Aβ-peptide induces an axonal loss in a reconstructed cortical-hippocampal network. Cortical (Cx) and hippocampal (Hi) neurons were cultured for 14 days in μFD chambers as in Figure 2. Hippocampal neurons and projecting cortical axons were immunodetected using anti-MAP2 (red), anti-α-tubulin (green). Cx chambers were treated with sham (a, Ø/ Ø) or 10 μM Aβ42 oligomers (b, Aβ /Ø) for 48 hours. Representative fluorescence micrographs from the distal hippocampal chamber receiving cortical fibers. Scale bar: 20 μM.