Figure 2

Axonal administration of NAD+, broad-spectrum caspase inhibitor, and JNK inhibitor reduces Aβ-induced axonal degeneration. Cortical (Cx) neurons were cultured for 12 days in μFD chambers as in Figure 1. Somatic Aβ peptide treatment (48 h) induced a strong axonal fragmentation, which could be reversed by pre-treatment of the axonal compartment with 5 mM NAD⁺, 50 μM z-VAD-fmk or 50 μM SP600125 (n = 5; *P < 0.01; **P < 0.001, ANOVA).