Figure 1
Motor and exploratory activity in the open field test is decreased in the two month-old Mcoln1 −/− mice. Data presented as mean values ± SEM for 8 wild-type (WT) and 13 Mcoln1 −/− (KO) one month-old and 17 wild-type and 18 Mcoln1 −/− two month-old mice. Each mouse, at either one or two months of age, was tested once in the open field and spontaneous activity during 10 min of exploration in the open-field arena was recorded. Two-way ANOVA analysis revealed: significant interaction between age and genotype in jump counts (F genotype × age = 6.96, p = 0.0271) (A) and jump time (F genotype × age = 5.36, p = 0.0409) (B); significant effects of age and genotype on vertical activity, expressed as rearing (F genotype = 6.16, p = 0.0155; F age = 38.09, p < 0.0001) (C), and vertical time (F genotype = 3.87, p > 0.05; F age = 38.7, p < 0.0001) (D), resting time (F genotype = 6.91, p = 0.0128; F age = 35.75, p < 0.0001) (E) and exploratory activity, expressed by percentage of central track length (F genotype = 11.15, p = 0.0087; F age = 8.69, p = 0.0197) (F), percentage of time spent in the center (F genotype = 9.99, p = 0.0111; F age = 10.12, p = 0.0106) (G) and the number of central zone entries (F genotype × age = 5.36, p = 0.0409) (H). Asterisks indicate significant difference between genotypes at given time-points as determined by post-hoc t-test with Bonferroni correction, p* - <0.05, ** - <0.01, *** - <0.001.