Figure 15

Assessment of Iba1 microglial activation 12 hrs post CpG ODN injection. Accumulation of Iba1 immunoreactive cells was not affected by acute injection of CpG ODN. Representative hippocampal images ( A , B ) and subsequent semiquantitative comparisons of Iba1 reactive microglia showed no group differences in both age cohorts ( C ). Scale bar, 100 µm. Note that double immunofluorescence labeling with CD45 (green) and Iba1 (red) did not reveal co-localization ( D ). Scale bar, 50 µm.