Table 3 Primary and secondary antibodies used for single labeling
Antigen | Antigen retrieval | Species | Final dilution | Source primary antibody | Secondary antibody | Source secondary antibody |
|---|---|---|---|---|---|---|
Human MBP | Tris-EDTA | Mouse | 1:100 | Boehringer, 1118099 | Biotinylated donkey-anti-mouse IgG | Jackson, 715-065-151 |
Human MHC-II | Citrate | Mouse | 1:100 | Gift, Clone LN3 | Biotinylated donkey-anti-mouse IgG | Jackson, 715-065-151 |
Human CCL2 | Citrate | Mouse | 1:200 | R&D Systems, MAB2791 | Biotinylated goat-anti-mouse IgG | Jackson, 115-065-146 |
Human CCR2 | Citrate | Rabbit | 1:1000 | Abcam, ab32144 | Biotinylated goat-anti-rabbit IgG | Jackson, 111-065-144 |