Figure 5

Cell type and activation state analysis of CNM genes. (A) Cell type-specificity of the CNM genes. CNM genes were categorized by cell type enrichment based on analysis of public data (see Materials and Methods). The distribution of genes in the categories is shown for CNM genes up-regulated (left) and down-regulated (right). (B) Assessment of microglia/monocyte activation and reactive astrocyte states in the discovery meta-analysis gene list. GSEA for custom gene sets for glial cell polarization states from isolated cell transcriptome analyses in the literature. Positive normalized enrichment scores indicate enrichment in neurodegeneration, while negative score indicate enrichment in control tissue. All enrichments are significant (p < 0.005), except for ALS microglia (down). ALS (up or down), amyotrophic lateral sclerosis, up-regulated or down-regulated genes (mouse model); LPS, lipopolysaccharide treated mouse, up-regulated genes; MCAO, middle cerebral artery occlusion mouse, up-regulated genes.