A neuronal function of the tumor suppressor protein merlin
© Schulz et al.; icensee BioMed Central Ltd. 2014
Received: 27 May 2014
Accepted: 30 June 2014
Published: 12 July 2014
Mutagenic loss of the NF2 tumor suppressor gene encoded protein merlin is known to provoke the hereditary neoplasia syndrome, Neurofibromatosis type 2 (NF2). In addition to glial cell-derived tumors in the PNS and CNS, disease-related lesions also affect the skin and the eyes. Furthermore, 60% of NF2 patients suffer from peripheral nerve damage, clinically referred to as peripheral neuropathy. Strikingly, NF2-associated neuropathy often occurs in the absence of nerve damaging tumors, suggesting tumor-independent events. Recent findings indicate an important role of merlin in neuronal cell types concerning neuromorphogenesis, axon structure maintenance and communication between axons and Schwann cells. In this review, we compile clinical and experimental evidences for the underestimated role of the tumor suppressor merlin in the neuronal compartment.
KeywordsNeurofibromatosis type 2 Merlin isoforms Peripheral neuropathy Axon-Schwann cell interaction Tumor development Intelligence
The hereditary disease Neurofibromatosis type 2
Mutations in the NF2 gene are causative for the autosomal-dominant disease Neurofibromatosis Type 2 (NF2). This rare multiple neoplasia syndrome affects about 1 in 25,000 live births []. However, recent population studies suggest that up to 1 in 300 people will develop a tumor with an underlying sporadic NF2 mutation during their lifetime []. The heritable NF2 disease is mainly characterized by the development of benign Schwann cell-derived tumors, called schwannomas, due to the mutagenic loss of the tumor suppressor merlin. The hallmark feature of NF2 is the bilateral occurrence of schwannomas at the eighth cranial nerve (vestibular schwannoma). These tumors regularly develop in close vicinity to the ‘Obersteiner-Redlich zone’ [] – the boundary between CNS and PNS – where the transition between Schwann cell and oligodendrocyte myelination takes place. Compressive effects of the schwannoma onto the vestibulo-cochlear nerve may subsequently result in loss of hearing and balance. In addition to vestibular schwannomas and schwannomas occurring within the spinal cord and along peripheral nerves, mutations in the NF2 gene are responsible for virtually all non-hereditary, sporadically occurring schwannomas and 50% of sporadic meningioma cases [].
However, NF2 is a clinical syndrome that presents with a variety of other clinical manifestations. In addition to tumors of various entities, NF2 patients suffer from disease-related lesions affecting the skin and the eyes (for detailed review see []). Most affected individuals will develop damage to peripheral nerves (peripheral neuropathy) in their lifetime, another common clinical feature in NF2. To date, the pathogenesis of NF2-related neuropathy is not completely understood. Taken together, due to a variety of organ systems being affected by NF2 disease, affected individuals may suffer from severe morbidity in addition to their tumor burden.
Mutations affecting the NF2 gene may become apparent through at least three kinds of different genetic alterations. Firstly, inherited mutations due to germline mutations result in the loss of one allele; these are accompanied by somatic alterations in the other allele, which cause the hereditary Neurofibromatosis Type 2. Secondly, sporadic schwannomas depend on the acquired somatic mutations in both alleles of the NF2 gene. Thirdly, as we will discuss later, NF2-related neuropathy may result from the loss of just one allele as a consequence of cell type-specific haploinsufficiency in neuronal cell types.
The tumor suppressor protein merlin, responsible for NF2, is ubiquitously expressed in all tissues during all periods of development []. Homozygous deletion of merlin in mice leads to embryonic failure, even before gastrulation []. Moreover, conditional ablation of merlin during embryogenesis results in a global tissue fusion defect [], indicating the importance of merlin from the earliest stage of development. While the role of merlin in glial cell types has been extensively characterized during both development and adulthood, the expression and function in non-tumor related tissues has only occasionally been subjected to mainstream NF2 research.
Microenvironment considerations have become a large field of interest in life science; no given cell type can be comprehensively considered without the context of its environment. Cells in direct or close vicinity influence their neighboring cells - effecting tissue homeostasis. Schwann cells, the origin for NF2-related tumors, are in tight and direct contact with axons-resulting in extensive inter-cellular crosstalk and provoking the hypothesis that axons and/or axon-derived signals, respectively, contribute to tumorigenic activity of Schwann cells. We propose that an exclusive focus on Schwann cell biology in NF2 research risks neglecting not only other high-prevalence symptoms, which occur in NF2 disease, but also potential microenvironmental issues that could contribute to NF2 tumorigenesis. For instance, peripheral neuropathy has been found to appear in individuals who bear mutations in just one merlin allele and lack a significant load of potentially compressive Schwann cell tumors []. This led us to the idea that merlin expressed in neurons might have functions unrelated to its tumor suppressor role in glial cells [].
The tumor suppressor protein merlin
The human NF2 gene on Chromosome 22q12.2 comprises 17 exons that encode for the 595 amino acid protein merlin; also known as schwannomin [,]. This actin-binding protein belongs to the ezrin–radixin–moesin (ERM) family of proteins that organizes and links membrane proteins to the cortical cytoskeleton []. Merlin mediates contact inhibition of proliferation in multiple cell types, including Schwann cells [] and is reported to target many signaling components to restrict proliferation []. For an extensive review of merlin effected pathways please see []. Moreover, the tumor suppressor merlin activity is suggested to take place in various cellular compartments, including the cell nucleus [,], at the plasma membrane [,], in endosomes [] and even in association with mitotic spindles during mitosis []. Although merlin interacts with a high number of different molecules (for detailed review see []) in different locations of the cell, we still conclude that part of merlin’s tumor suppressor activity is at the plasma membrane - mediating contact inhibition of proliferation by regulating several small GTPases like Ras or the Rho GTPase family, as well as the Hippo pathway [].
Rho GTPases in neuronal cell types
GTPase proteins are molecular switches that regulate many important processes in the cell, including the organization of the actin cytoskeleton []. By provoking local actin rearrangements, the protein family of Rho GTPases is essential for the development of highly polarized cells like neurons []. Regulators of these small GTPases are therefore of special interest in the broad field of neuromorphogenesis. Merlin has often been shown to exert its various functions through Rho GTPases by determining their activation state [,,]. While GDP-bound molecules are considered to be inactive, GTP-bound proteins actively act on their downstream targets. Considering the significant importance of small GTPases in neuromorphogenesis, merlin, as well as other regulators of small GTPase activity, are plausible candidates for involvement in the vastly complex process of neuronal shape determination. Significantly, mutations in regulators and effectors of Rho GTPases have been associated with diseases of the nervous system, including mental retardation and motor neuron diseases [].
The appearance of merlin isoforms
The human gene NF2 and its close homologue the murine gene Nf2 are subject to alternative splicing []. By far the most abundant isoforms are isoform 1 (595 aa) and isoform 2 (590 aa), which differ in their last 11 and 16 amino acids, respectively []. While merlin isoform 1 contains exon 17 instead of exon 16, merlin isoform 2 contains the stop codon bearing exon 16, which results in a C-terminal truncated protein [].
The altered C-terminus of isoform 2 is hydrophilic and positively charged, while the isoform 1 C-terminus is much less hydrophilic and has no net charge. As a consequence isoform 1 C-terminus binds strongly to the N-terminal FERM domain, while the isoform 2 C-terminus shows only weak binding []. Apparently, both C-termini can interact with each other, proposing the formation of isoform hetero-dimers []. Due to the structural and charge differences in their very C-terminus, the two main merlin isoforms are likely to have different binding partners in cases where the C-terminus is necessary for protein-protein interactions. So far, only syntenin, an adaptor protein involved in the subcellular trafficking of receptors, has been shown to specifically interact with the C-terminus of isoform 1 []. Although merlin has been implicated in receptor trafficking [], the functional consequence of a specific merlin isoform 1 interaction has not been described.
To date, it remains controversial as to whether both major merlin isoforms exert a tumor suppressive function. No pathogenic mutation that specifically hits one isoform of NF2 has been described; tumorigenic mutations always inactivate both isoforms [,]. The only data on functional differences of the two merlin isoforms comes from in vitro studies: Of the two major merlin isoforms, only isoform 1 was originally thought to have proliferation suppressive potential [,]. However, more recent studies suggest that both isoforms have equal proliferation inhibiting functions and so far act similarly in most analyzed assays [–]. Despite structural differences in the C-terminus, it is reasonable to assume that both isoforms have partially overlapping functions whenever the homologous N-terminus is involved in the regulation of downstream pathways. Furthermore, in an intact cellular system, differences between the two major merlin isoforms may be due to their potential sites of activity - which could be determined by specific isoform binding partners targeting them to distinct cellular and subcellular localizations []. Clearly, greater focus and effort is required to identify and catalogue merlin isoforms and their functions. Early studies investigating the spatiotemporal expression pattern of NF2/Nf2 isoforms suggest that merlin plays a pivotal role in neuronal tissue, especially during development. High NF2 expression was found in brains of humans [,] as well as rodents [,]. Interestingly, Gutmann et al. reported an increase in isoform 2 expression during neuronal maturation in the cerebral cortex and cerebellum. Additionally, compared to embryonic tissue, neuronal tissue was one of the few organs to retain high expression levels of Nf2 in adult rats []. However, a relevant function of merlin isoform 2, in neurons or other cell types, remained elusive. Only recently have we been able to decipher a unique function for merlin isoform 2; wherein this specific isoform is located and operates in the axonal compartment of neurons [].
Expression pattern of merlin in neuronal cells
Although merlin has been studied primarily in glial cells, due to loss of merlin primarily attracting attention by causing benign tumors, several lines of evidence now support additional and functional roles of merlin in neurons. To date, several studies have reported protein expression of merlin in different types of neuronal cells of both the PNS and CNS.
Through different imaging techniques such as immunohistochemistry and in-situ-hybridization, merlin has been detected in sciatic nerve axons [], in neurons that belong to autonomic ganglia in the intestinal tract [] and in dorsal root ganglion cells of the PNS [].
In the CNS, merlin appears in motor neurons of the spinal cord [], cortical neurons [,,], hippocampal neurons [,], neurons of cranial nerve ganglia [] and in cerebellar Purkinje cells [,,,]. Particularly in Purkinje cells of the cerebellum, merlin could be functionally associated with neuromorphogenesis and dendritic arborization through the regulation of the small GTPase Rac1 []. Furthermore, embryonic expression of merlin in neural stem cells could be demonstrated in neuroepithelial cells of the neural tube, as well as in the ventricular and subventricular zone of the developing brain [,]. The analysis of brain tissue and neuronal progenitor cell (NPC) cultures showed consistently that merlin is predominantly present in neurons [].
On the subcellular level, neuronal merlin was found to be expressed in dendrites [], in axons [,], in the cytoplasm [,] and in neuronal synaptic junctions []. Conclusively, there is now consistent and broad evidence for a neuronal expression of the tumor suppressor merlin in both rodent and human tissue. However, little is known yet to explain merlin’s function in each cell type. This discrepancy clearly needs to be addressed in the future.
Polyneuropathy in NF2 patients
Besides the development of multiple gliogenic tumors affecting both the PNS and CNS, many NF2 patients will develop peripheral neuropathy during their lifetime. Affected individuals can suffer from stocking-like hypoalgesia (reduced sensitivity to pain) and hypesthesia (decreased tactile sensibility) as well as loss of vibration sense (pallhypesthesia). Patients may also present with a distal reflex loss that can be followed by a slow but progressive distal muscle atrophy and paresis in later stages of the disease []. Usually, peripheral neuropathy can occur as a rather local phenomenon (mononeuropathy simplex or multiplex) or a more generalized event (polyneuropathy).
Indeed, the exact proportion of NF2 sufferers who develop peripheral nerve damage remains obscure, as prevalence numbers vary largely. In a huge clinical study, peripheral nerve lesions unrelated to tumor masses were observed in 6% of patients suffering from NF2 []. Another investigation, with primary focus on NF2-related neuropathy, found that clinical signs manifesting as peripheral neuropathy occurred in 47% of investigated patients []. Further electrophysiological examination even revealed evidence of neuropathy in 67% of those individuals.
The general observation that many NF2 patients present with areflexia, which cannot be completely explained by the actual tumor load, suggests that subclinical or masked neuropathy is potentially underdiagnosed in NF2 disease [].
Electrophysiological methods as a diagnostic tool for neuromuscular diseases
Electrophysiological measurements are an indispensable tool for investigating the functional integrity of peripheral nerves in both the clinical and laboratory environment []. In humans, a large number of neuromuscular disorders and neuropathies diagnostically rely on electrophysiological measurements. By measuring nerve properties as conduction velocity or potential amplitudes of the signal, it is possible to characterize the origin of peripheral nerve diseases. The nerve conduction velocity is highly dependent on rapid signal propagation enabled by myelination. Therefore, demyelinating processes generally show decreased conduction velocities. When significantly reduced, the compound motor action potential (CMAP) – correlating with the number of functional axons – is an indicator for axonal damage. Hence, by means of electrophysiological methods, the etiology of peripheral nerve damage can be discriminated; such as for hereditary neuropathies, diabetic neuropathy, chronic inflammatory demyelinating polyneuropathies (CIDP) or metabolic neuropathies.
Exploring the pathogenesis of NF2-related neuropathy
Originally, schwannomas were held to primarily account for observable neuropathic symptoms developing in the course of NF2 []. Schwannomas can occur within the spinal cord, on spinal nerve roots, along peripheral nerves and around cranial nerves – with the vestibular nerve being the most frequently involved cranial nerve. The localization of a given tumor naturally determines the presenting features and symptoms of an individual, e.g. affections of a spinal nerve root by a tumor may cause motoric and sensory problems that are clearly related to its innervation area. However, for the most part, clinical signs of neuropathy appear independently from the site of peripheral nerve schwannomas. In single NF2 patients, polyneuropathy even developed years before other NF2-related symptoms, like tumors, became evident [].
Although benign in nature, schwannomas are thought to produce pain and other symptoms by compressive effects, thereby impairing axonal integrity in a given nerve. However, the clinical appearance of neuropathy can hardly be explained by the tumor burden alone. Concretely, in some NF2 patients suffering from polyneuropathy, muscle weakness occurs without significant spinal or peripheral nerve tumor burden, suggesting that factors other than gross tumor growth might be responsible for this disorder []. Besides, surgical resection of gross tumor load along peripheral nerves often lacks a beneficial outcome for affected individuals in terms of neuropathic symptoms []. Furthermore, NF2-associated polyneuropathy typically involves more than two peripheral nerves and predominantly affects extremities in a distal and symmetric fashion [,,], suggesting a systemic rather than local issue. Thus, tumorlets - hyperproliferative Schwann cells – are also unlikely to explain the complete etiology of peripheral neuropathy in these patients []. However, a high-resolution MRI study – aiming to link tumor load with severity of polyneuropathy in NF2 patients – indicated that non-compressive fascicular microlesions along peripheral nerves, correlated with severity of clinical symptoms of NF2-related neuropathy. Apart from that, compressive tumor macrolesions were absent in most neuropathy-affected extremities [].
Both neuropathological and electrophysiological investigations initially suggested that NF2-related polyneuropathy might develop independently of large solitary schwannomas []. Hagel et al. provided evidence for an axon-intrinsic pathogenesis of neuropathy in sural nerve biopsies indicated by pathological reduction of nerve fiber densities, accompanied by diffuse proliferation of Schwann cells. Onion bulbs, pathological indicators of repetitive de-/remyelination, were just seen in a subset of investigated patients []. Furthermore, by determining nerve conduction properties, two studies were able to show that NF2-related peripheral neuropathy is commonly of axonal origin [,]. In the majority of cases, the nerve conduction velocity appeared normal (above the reference levels of the tested nerves), while CMAP values were markedly decreased – a diagnostic combination suggestive of axonal neuropathy.
Merlin in axon-Schwann cell interactions
The implication of merlin in prevention of Schwann cell tumorigenesis has been extensively studied [,]. NF2-related schwannomas are encapsulated tumors composed almost entirely of Schwann cells perched on, but not commingled with, normal nerve bundles []. However, the benign dignity of NF2-associated Schwann cell-derived tumors is accompanied by sparse response to classical chemotherapy [].
Importantly, the role of merlin in Schwann cells is not just restricted to its tumor suppressive function. It has been reported to play a critical role in the control of Schwann cell numbers and is necessary for the correct organization and regulation of axo-glial heterotypic contacts []. Consistently, merlin in Schwann cells has been reported to promote their alignment along axons and ultimately influences myelin segment length [].
Generally, the behavior of Schwann cells is strictly under the control of axonal signals, both during development and in adulthood []. As such, Schwann cell actions should not be assessed solely by endogenous Schwann cell signaling pathways, but rather with respect to the influence of axons and vice versa. Signals from axons regulate the intimate communication of Schwann cells with axons of the PNS, provide proliferative and survival signals, and determine the polarization and differentiation programs to either non-myelinating or myelinating phenotypes [,]. Moreover, axonal damage triggers rapid Schwann cell de-differentiation and this is accompanied by myelin breakdown, Schwann cell detachment from axons and subsequent proliferation []. Typically, patients with NF2 present with different types of benign Schwann cell tumors, in which most Schwann cells lose contact with axons [,]. Focusing on the pathogenesis of polyneuropathy affecting NF2 patients, Sperfeld and colleagues [] also suggested that the nerve damaging disease could possibly occur because Schwann cells can no longer adhere properly to the axons. This underlines the importance of the microenvironment of peripheral nerves, where damage to one cell type invariably leads to pathophysiological changes in the other [].
Binding or interaction partners of neuronal merlin
Cell type; species
DRG, sciatic nerve lysates (mouse)
Riβ (PKA subunit)
brain lysates (rat)
brain extracts (rat)
neuroblastoma cells (mouse)
Cell lysate from primary neurons (mouse)
Sciatic nerve lysates (mouse)
In accordance, human sural nerve biopsies taken from NF2 patients suffering from polyneuropathy display a strong and consistent reduction of Nrg1 type III. This is accompanied by a compensatory up-regulation of ErbB2 expression on Schwann cells; as analyzed in mice bearing neuron-specific merlin knockout as well as NF2 patient samples. Notably, the expression abnormalities of both Nrg1 type III and ErbB2 receptor appear to be very specific to NF2 disease and much more pronounced than in other axonal types of neuropathies [].
ErbB2/ErbB3 heterodimers are neuregulin receptors which are required for SC development []. In mature peripheral nerves, Nrg1 participates in the regeneration and re-myelination of injured myelinated fibers, processes that involve SC de-differentiation, proliferation and re-differentiation to a myelinating phenotype []. Interestingly, ErbB2/3 receptor overexpression has been linked to the pathogenesis of one type of demyelinating neuropathy occurring in the course of Charcot-Marie-Tooth disease type 1 [], which raises the possibility of Schwann cell-autonomous effects on the development of neuropathies.
Remarkably, the loss of merlin in primary Schwann cells is associated with elevated levels of ErbB receptors []. Furthermore, merlin in Schwann cells interacts with CD44 [], a membrane glycoprotein that enhances neuregulin-induced ErbB2 phosphorylation []. Concerning the regulation of ErbB2/3 receptor expression, merlin obviously has synergistic functions in neurons and Schwann cells, arguing for a holistic function of merlin in both cellular compartments of peripheral nerves. Consequently, ErbB2/3 receptor overexpression has been identified as a potential target for NF2 therapy [], using the monoclonal antibody Trastuzumab [] or the tyrosine kinase inhibitor Lapatinib [].
Axons are thought to maintain Schwann cells in a differentiated state during adulthood, to ensure the correct functioning of the nerve [,]. It is therefore reasonable to assume that misregulation of axon surface proteins–essential for Schwann cell alignment and differentiation – could contribute to the initial events in tumor development. Notably, a comparable pathogenesis is likely to occur in the related tumor syndrome Neurofibromatosis Type 1 (NF1); possibly the most common inherited disorder caused by a single gene, which is characterized by the development of multiple neurofibromas. Since heterotypic cell–cell contacts control cell proliferation and suppress tumorigenesis [], the loss of Schwann cell contact to axons is a frequent and important early event in tumor development for these highly heterotypic benign tumors of the peripheral nerve sheath. Joseph et al. [] could additionally show that NF1-related tumors arise from differentiated glial cells instead of undifferentiated neural crest cells. These results further support the hypothesis that Schwann cell detachment from axons is an important early event in tumor development – a mechanism neuronally expressed merlin could be also involved in concerning NF2 disease. Taken together, new findings on merlin in the neuronal compartment suggest pathogenesis of NF2 disease, wherein the NF2 gene encoded protein has cell type-dependent functions in order to prevent tumor formation.
In a recent study [] the impressive number of proteins merlin can interact with was further expanded (for detailed review see []). More precisely, merlin has now been reported to associate with all three classes of cytoskeletal elements; namely actin filaments [], microtubules [,] and intermediate filaments []. This connection underlines merlin’s fascinating role as a versatile cytoskeleton associated molecule involved in a vast variety of signaling events. This makes merlin a highly enigmatic and extraordinary tumor suppressor. However, the contribution of merlin splice variants may play an additional role for the interaction with multiple proteins in a large variety of different cell types.
According to Knudson’s two-hit-hypothesis, merlin acts as a classical tumor suppressor. Interestingly, inactivating mutations of merlin seem to have tissue-dependent diverse effects. Loss of heterozygosity, the functional loss of one gene allele in which the other allele was already inactivated, is known to be crucial for merlin owed tumor formation in Schwann cells [,]. However, deficient effects due to loss of merlin can already be detected in neurons where only one mutation is verifiable []; thus explaining why polyneuropathy in NF2 patients is frequently found in the absence of compressive tumors and may even appear chronologically earlier. In line with this notion, axons of mice heterozygous for merlin isoform 2 mutations show functional and morphological abnormalities []. However, despite the clear relevance of merlin in neuronal cells of the PNS, a functional role for merlin in CNS neurons remains elusive.
Grönholm et al. [] provided a first functional hint for neuronally expressed merlin in the CNS. It was shown to be the first known binding partner of Riβ, a regulatory subunit of protein kinase A (PKA), which is evidently implicated in learning-related functions []. Consistently, Wassink and colleagues [] reported that merlin is a candidate gene for the development of autism spectrum disorder (ASD), which has been shown to be associated with dendritic spine abnormalities []. Because dendritic spine morphology is in turn highly susceptible to the activation state of small GTPases [], an impact of merlin on spine morphology and/or plasticity is very likely but, as yet, defies characterization. In line with this hypothesis, the loss of merlin in neural progenitor cells results in severe reduction in hippocampus size []; the implications of which in learning and memory acquisition are indisputable. However, despite merlin’s theoretical implication in learning and memory acquisition, no study has ever suggested changes in NF2 patients’ intelligence or cognitive performance. If such potential effects on learning and memory were to exist, these could be rather subtle and/or hidden by the vast environmental noise that envelops human intelligence.
How can future disease models and considerations regarding NF2 pathogenesis better emphasize the importance of the nerve microenvironment?
Does the loss of neuronal merlin and its influence on Schwann cell behavior impair peripheral nerve regeneration following injury?
Does merlin deficiency in neurons contribute to NF2-related schwannoma formation?
Is the downstream signaling of axonal merlin isoform 2 – involving RhoA and ROCK – relevant for other hereditary neuropathies whose mechanisms have yet to be deciphered?
Are Schwann cell-autonomous effects of merlin due to loss-of-heterozygosity sufficient to promote NF2-related neuropathy without any disturbances in the axonal compartment?
Are there alterations in cognitive performances in merlin-deficient animals and patients suffering from the NF2 disease?
What are the specific functions of the two major merlin isoforms? Is there a cell type-specific expression? With regard to the variety of different merlin functions, which merlin isoforms can compensate for each other?
The authors would like thank to Prof. Stephan Baader for critical reading of the manuscript and valuable suggestions. This work was supported by SFB 604, DFG MO 1421/2-1 and Krebshilfe 107089. A.S. is a recipient of a Young Investigator Award from the Children’s Tumor Foundation.
- Baser ME, R Evans DG, Gutmann DH: Neurofibromatosis 2. Curr Opin Neurol 2003, 16: 27–33. doi:10.1097/01.wco.0000053583.70044.ab 10.1097/00019052-200302000-00004View ArticlePubMedGoogle Scholar
- Evans DG, Kalamarides M, Hunter-Schaedle K, Blakeley J, Allen J, Babovic-Vuskanovic D, Belzberg A, Bollag G, Chen R, DiTomaso E, Golfinos J, Harris G, Jacob A, Kalpana G, Karajannis M, Korf B, Kurzrock R, Law M, McClatchey A, Packer R, Roehm P, Rubenstein A, Slattery W 3rd, Tonsgard JH, Welling DB, Widemann B, Yohay K, Giovannini M: Consensus recommendations to accelerate clinical trials for neurofibromatosis type 2. Clin Cancer Res 2009, 15: 5032–5039. doi:10.1158/1078–0432.CCR-08–3011 10.1158/1078-0432.CCR-08-3011View ArticlePubMedPubMed CentralGoogle Scholar
- Roosli C, Linthicum FH Jr, Cureoglu S, Merchant SN: What is the site of origin of cochleovestibular schwannomas? Audiol Neurootol 2012, 17: 121–125. doi:10.1159/000331394 10.1159/000331394View ArticlePubMedGoogle Scholar
- Lee JH, Sundaram V, Stein DJ, Kinney SE, Stacey DW, Golubic M: Reduced expression of schwannomin/merlin in human sporadic meningiomas. Neurosurgery 1997, 40: 578–587.PubMedGoogle Scholar
- Asthagiri AR, Parry DM, Butman JA, Kim HJ, Tsilou ET, Zhuang Z, Lonser RR: Neurofibromatosis type 2. Lancet 2009, 373: 1974–1986. doi:10.1016/S0140–6736(09)60259–2 10.1016/S0140-6736(09)60259-2View ArticlePubMedPubMed CentralGoogle Scholar
- Gutmann DH, Wright DE, Geist RT, Snider WD: Expression of the neurofibromatosis 2 (NF2) gene isoforms during rat embryonic development. Hum Mol Genet 1995, 4: 471–478. 10.1093/hmg/4.3.471View ArticlePubMedGoogle Scholar
- McClatchey AI, Saotome I, Ramesh V, Gusella JF, Jacks T: The Nf2 tumor suppressor gene product is essential for extraembryonic development immediately prior to gastrulation. Genes Dev 1997, 11: 1253–1265. 10.1101/gad.11.10.1253View ArticlePubMedGoogle Scholar
- McLaughlin ME, Kruger GM, Slocum KL, Crowley D, Michaud NA, Huang J, Magendantz M, Jacks T: The Nf2 tumor suppressor regulates cell-cell adhesion during tissue fusion. Proc Natl Acad Sci U S A 2007, 104: 3261–3266. doi:10.1073/pnas.0700044104 10.1073/pnas.0700044104View ArticlePubMedPubMed CentralGoogle Scholar
- Hanemann CO, Diebold R, Kaufmann D: Role of NF2 haploinsufficiency in NF2-associated polyneuropathy. Brain Pathol 2007, 17: 371–376. doi:10.1111/j.1750–3639.2007.00086.x 10.1111/j.1750-3639.2007.00086.xView ArticlePubMedGoogle Scholar
- Ramesh V: Merlin and the ERM proteins in Schwann cells, neurons and growth cones. Nat Rev Neurosci 2004, 5: 462–470. doi:10.1038/nrn1407 10.1038/nrn1407View ArticlePubMedGoogle Scholar
- Rouleau GA, Merel P, Lutchman M, Sanson M, Zucman J, Marineau C, Hoang-Xuan K, Demczuk S, Desmaze C, Plougastel B, Pulst SM, Lenoir G, Bijlsma E, Fashold R, Dumanski J, de Jong P, Parry D, Eldrige R, Aurias A, Delattre O, Thomas G: Alteration in a new gene encoding a putative membrane-organizing protein causes neuro-fibromatosis type 2. Nature 1993, 363: 515–521. doi:10.1038/363515a0 10.1038/363515a0View ArticlePubMedGoogle Scholar
- Trofatter JA, MacCollin MM, Rutter JL, Murrell JR, Duyao MP, Parry DM, Eldridge R, Kley N, Menon AG, Pulaski K, Haase VH, Ambrose CM, Munroe D, Bove C, Haines JL, Martuza RL, MacDonald ME, Seizinger BR, Short MP, Buckler AJ, James F, Gusella JF: A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor. Cell 1993, 72: 791–800. 10.1016/0092-8674(93)90406-GView ArticlePubMedGoogle Scholar
- McClatchey AI, Fehon RG: Merlin and the ERM proteins–regulators of receptor distribution and signaling at the cell cortex. Trends Cell Biol 2009, 19: 198–206. doi:10.1016/j.tcb.2009.02.006 10.1016/j.tcb.2009.02.006View ArticlePubMedPubMed CentralGoogle Scholar
- Morrison H, Sherman LS, Legg J, Banine F, Isacke C, Haipek CA, Gutmann DH, Ponta H, Herrlich P: The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44. Genes Dev 2001, 15: 968–980. doi:10.1101/gad.189601 10.1101/gad.189601View ArticlePubMedPubMed CentralGoogle Scholar
- Morrison H, Sperka T, Manent J, Giovannini M, Ponta H, Herrlich P: Merlin/neurofibromatosis type 2 suppresses growth by inhibiting the activation of Ras and Rac. Cancer Res 2007, 67: 520–527. doi:10.1158/0008–5472.CAN-06–1608 10.1158/0008-5472.CAN-06-1608View ArticlePubMedGoogle Scholar
- Li W, Cooper J, Karajannis MA, Giancotti FG: Merlin: a tumour suppressor with functions at the cell cortex and in the nucleus. EMBO Rep 2012, 13: 204–215. doi:10.1038/embor.2012.11 10.1038/embor.2012.11View ArticlePubMedPubMed CentralGoogle Scholar
- Muranen T, Gronholm M, Renkema GH, Carpen O: Cell cycle-dependent nucleocytoplasmic shuttling of the neurofibromatosis 2 tumour suppressor merlin. Oncogene 2005, 24: 1150–1158. doi:10.1038/sj.onc.1208283 10.1038/sj.onc.1208283View ArticlePubMedGoogle Scholar
- Li W, You L, Cooper J, Schiavon G, Pepe-Caprio A, Zhou L, Ishii R, Giovannini M, Hanemann CO, Long SB, Erdjument-Bromage H, Zhou P, Tempst P, Giancotti FG: Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin ligase CRL4(DCAF1) in the nucleus. Cell 2010, 140: 477–490. doi:10.1016/j.cell.2010.01.029 10.1016/j.cell.2010.01.029View ArticlePubMedPubMed CentralGoogle Scholar
- Mani T, Hennigan RF, Foster LA, Conrady DG, Herr AB, Ip W: FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function. Mol Cell Biol 2011, 31: 1983–1996. doi:10.1128/MCB.00609–10 10.1128/MCB.00609-10View ArticlePubMedPubMed CentralGoogle Scholar
- Scoles DR, Huynh DP, Chen MS, Burke SP, Gutmann DH, Pulst SM: The neurofibromatosis 2 tumor suppressor protein interacts with hepatocyte growth factor-regulated tyrosine kinase substrate. Hum Mol Genet 2000, 9: 1567–1574. 10.1093/hmg/9.11.1567View ArticlePubMedGoogle Scholar
- Muranen T, Gronholm M, Lampin A, Lallemand D, Zhao F, Giovannini M, Carpen O: The tumor suppressor merlin interacts with microtubules and modulates Schwann cell microtubule cytoskeleton. Hum Mol Genet 2007, 16: 1742–1751. doi:10.1093/hmg/ddm122 10.1093/hmg/ddm122View ArticlePubMedGoogle Scholar
- Scoles DR: The merlin interacting proteins reveal multiple targets for NF2 therapy. Biochim Biophys Acta 2008, 1785: 32–54. doi:10.1016/j.bbcan.2007.10.001PubMedGoogle Scholar
- Yin F, Yu J, Zheng Y, Chen Q, Zhang N, Pan D: Spatial organization of Hippo signaling at the plasma membrane mediated by the tumor suppressor Merlin/NF2. Cell 2013, 154: 1342–1355. doi:10.1016/j.cell.2013.08.025 10.1016/j.cell.2013.08.025View ArticlePubMedGoogle Scholar
- Hall A: G proteins and small GTPases: distant relatives keep in touch. Science 1998, 280: 2074–2075. 10.1126/science.280.5372.2074View ArticlePubMedGoogle Scholar
- Luo L: Rho GTPases in neuronal morphogenesis. Nat Rev Neurosci 2000, 1: 173–180. doi:10.1038/35044547 10.1038/35044547View ArticlePubMedGoogle Scholar
- Schulz A, Geissler KJ, Kumar S, Leichsenring G, Morrison H, Baader SL: Merlin inhibits neurite outgrowth in the CNS. J Neurosci 2010, 30: 10177–10186. doi:10.1523/JNEUROSCI.0840–10.2010 10.1523/JNEUROSCI.0840-10.2010View ArticlePubMedGoogle Scholar
- Flaiz C, Kaempchen K, Matthies C, Hanemann CO: Actin-rich protrusions and nonlocalized GTPase activation in Merlin-deficient schwannomas. J Neuropathol Exp Neurol 2007, 66: 608–616. doi:10.1097/nen.0b013e318093e555 10.1097/nen.0b013e318093e555View ArticlePubMedGoogle Scholar
- Nadif Kasri N, Van Aelst L: Rho-linked genes and neurological disorders. Pflugers Arch 2008, 455: 787–797. doi:10.1007/s00424–007–0385–1 10.1007/s00424-007-0385-1View ArticlePubMedGoogle Scholar
- Golovnina K, Blinov A, Akhmametyeva EM, Omelyanchuk LV, Chang LS: Evolution and origin of merlin, the product of the Neurofibromatosis type 2 (NF2) tumor-suppressor gene. BMC Evol Biol 2005, 5: 69. doi:10.1186/1471–2148–5-69 10.1186/1471-2148-5-69View ArticlePubMedPubMed CentralGoogle Scholar
- Scoles DR, Huynh DP, Morcos PA, Coulsell ER, Robinson NG, Tamanoi F, Pulst SM: Neurofibromatosis 2 tumour suppressor schwannomin interacts with betaII-spectrin. Nat Genet 1998, 18: 354–359. doi:10.1038/ng0498–354 10.1038/ng0498-354View ArticlePubMedGoogle Scholar
- Meng JJ, Lowrie DJ, Sun H, Dorsey E, Pelton PD, Bashour AM, Groden J, Ratner N, Ip W: Interaction between two isoforms of the NF2 tumor suppressor protein, merlin, and between merlin and ezrin, suggests modulation of ERM proteins by merlin. J Neurosci Res 2000, 62: 491–502. 10.1002/1097-4547(20001115)62:4<491::AID-JNR3>3.0.CO;2-DView ArticlePubMedGoogle Scholar
- Jannatipour M, Dion P, Khan S, Jindal H, Fan X, Laganiere J, Chishti AH, Rouleau GA: Schwannomin isoform-1 interacts with syntenin via PDZ domains. J Biol Chem 2001, 276: 33093–33100. doi:10.1074/jbc.M105792200 10.1074/jbc.M105792200View ArticlePubMedGoogle Scholar
- Lallemand D, Manent J, Couvelard A, Watilliaux A, Siena M, Chareyre F, Lampin A, Niwa-Kawakita M, Kalamarides M, Giovannini M: Merlin regulates transmembrane receptor accumulation and signaling at the plasma membrane in primary mouse Schwann cells and in human schwannomas. Oncogene 2009, 28: 854–865. doi:10.1038/onc.2008.427 10.1038/onc.2008.427View ArticlePubMedGoogle Scholar
- Jacoby LB, MacCollin M, Barone R, Ramesh V, Gusella JF: Frequency and distribution of NF2 mutations in schwannomas. Genes Chromosomes Cancer 1996, 17: 45–55. doi:10.1002/(SICI)1098–2264(199609)17:1<45::AID-GCC7>3.0.CO;2–2 10.1002/(SICI)1098-2264(199609)17:1<45::AID-GCC7>3.0.CO;2-2View ArticlePubMedGoogle Scholar
- Baser ME, Kuramoto L, Woods R, Joe H, Friedman JM, Wallace AJ, Ramsden RT, Olschwang S, Bijlsma E, Kalamarides M, Papi L, Kato R, Carroll J, Lazaro C, Joncourt F, Parry DM, Rouleau GA, Evans DG: The location of constitutional neurofibromatosis 2 (NF2) splice site mutations is associated with the severity of NF2. J Med Genet 2005, 42: 540–546. doi:10.1136/jmg.2004.029504 10.1136/jmg.2004.029504View ArticlePubMedPubMed CentralGoogle Scholar
- Sherman L, Xu HM, Geist RT, Saporito-Irwin S, Howells N, Ponta H, Herrlich P, Gutmann DH: Interdomain binding mediates tumor growth suppression by the NF2 gene product. Oncogene 1997, 15: 2505–2509. doi:10.1038/sj.onc.1201418 10.1038/sj.onc.1201418View ArticlePubMedGoogle Scholar
- Gutmann DH, Sherman L, Seftor L, Haipek C, Hoang Lu K, Hendrix M: Increased expression of the NF2 tumor suppressor gene product, merlin, impairs cell motility, adhesionand spreading. Hum Mol Genet 1999, 8: 267–275. doi:ddc028 10.1093/hmg/8.2.267View ArticlePubMedGoogle Scholar
- James MF, Han S, Polizzano C, Plotkin SR, Manning BD, Stemmer-Rachamimov AO, Gusella JF, Ramesh V: NF2/merlin is a novel negative regulator of mTOR complex 1, and activation of mTORC1 is associated with meningioma and schwannoma growth. Mol Cell Biol 2009, 29: 4250–4261. doi:10.1128/MCB.01581–08 10.1128/MCB.01581-08View ArticlePubMedPubMed CentralGoogle Scholar
- Laulajainen M, Melikova M, Muranen T, Carpen O, Gronholm M: Distinct overlapping sequences at the carboxy-terminus of merlin regulate its tumour suppressor and morphogenic activity. J Cell Mol Med 2012, 16: 2161–2175. doi:10.1111/j.1582–4934.2012.01525.x 10.1111/j.1582-4934.2012.01525.xView ArticlePubMedPubMed CentralGoogle Scholar
- Zhan Y, Modi N, Stewart AM, Hieronimus RI, Liu J, Gutmann DH, Chadee DN: Regulation of mixed lineage kinase 3 is required for Neurofibromatosis-2-mediated growth suppression in human cancer. Oncogene 2011, 30: 781–789. doi:10.1038/onc.2010.453 10.1038/onc.2010.453View ArticlePubMedGoogle Scholar
- Gavilan HS, Kulikauskas RM, Gutmann DH, Fehon RG: In Vivo Functional Analysis of the Human NF2 Tumor Suppressor Gene in Drosophila. PLoS One 2014, 9: e90853. doi:10.1371/journal.pone.0090853 10.1371/journal.pone.0090853View ArticlePubMedPubMed CentralGoogle Scholar
- Bianchi AB, Hara T, Ramesh V, Gao J, Klein-Szanto AJ, Morin F, Menon AG, Trofatter JA, Gusella JF, Seizinger BR, Kley N: Mutations in transcript isoforms of the neurofibromatosis 2 gene in multiple human tumour types. Nat Genet 1994, 6: 185–192. doi:10.1038/ng0294–185 10.1038/ng0294-185View ArticlePubMedGoogle Scholar
- Schmucker B, Tang Y, Kressel M: Novel alternatively spliced isoforms of the neurofibromatosis type 2 tumor suppressor are targeted to the nucleus and cytoplasmic granules. Hum Mol Genet 1999, 8: 1561–1570. 10.1093/hmg/8.8.1561View ArticlePubMedGoogle Scholar
- Huynh DP, Tran TM, Nechiporuk T, Pulst SM: Expression of neurofibromatosis 2 transcript and gene product during mouse fetal development. Cell Growth Differ 1996, 7: 1551–1561.PubMedGoogle Scholar
- Schulz A, Baader SL, Niwa-Kawakita M, Jung MJ, Bauer R, Garcia C, Zoch A, Schacke S, Hagel C, Mautner VF, Hanemann CO, Dun XP, Parkinson DB, Weis J, Schroder JM, Gutmann DH, Giovannini M, Morrison H: Merlin isoform 2 in neurofibromatosis type 2-associated polyneuropathy. Nat Neurosci 2013, 16: 426–433. doi:10.1038/nn.3348 10.1038/nn.3348View ArticlePubMedGoogle Scholar
- den Bakker MA, Vissers KJ, Molijn AC, Kros JM, Zwarthoff EC, van der Kwast TH: Expression of the neurofibromatosis type 2 gene in human tissues. J Histochem Cytochem 1999, 47: 1471–1480. 10.1177/002215549904701113View ArticlePubMedGoogle Scholar
- Claudio JO, Lutchman M, Rouleau GA: Widespread but cell type-specific expression of the mouse neurofibromatosis type 2 gene. Neuroreport 1995, 6: 1942–1946. 10.1097/00001756-199510020-00028View ArticlePubMedGoogle Scholar
- Wang Q, Zhou D, Yan B, Lin X, Zhang SF: [Expression of Merlin in cortex of temporal lobe and in hippocampal CA1 region of the Kindling Model of Epilepsy induced by corciaria lactone in rats]. Sichuan Da Xue Xue Bao Yi Xue Ban 2006, 37: 115–117.PubMedGoogle Scholar
- Chen Y, Yu P, Lu D, Tagle DA, Cai T: A novel isoform of beta-spectrin II localizes to cerebellar Purkinje-cell bodies and interacts with neurofibromatosis type 2 gene product schwannomin. J Mol Neurosci 2001, 17: 59–70. doi:10.1385/JMN:17:1:59 10.1385/JMN:17:1:59View ArticlePubMedGoogle Scholar
- Lavado A, He Y, Pare J, Neale G, Olson EN, Giovannini M, Cao X: Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators. Development 2013, 140: 3323–3334. doi:10.1242/dev.096537 10.1242/dev.096537View ArticlePubMedPubMed CentralGoogle Scholar
- Gronholm M, Teesalu T, Tyynela J, Piltti K, Bohling T, Wartiovaara K, Vaheri A, Carpen O: Characterization of the NF2 protein merlin and the ERM protein ezrin in human, rat, and mouse central nervous system. Mol Cell Neurosci 2005, 28: 683–693. doi:10.1016/j.mcn.2004.11.014 10.1016/j.mcn.2004.11.014View ArticlePubMedGoogle Scholar
- Seo PS, Quinn BJ, Khan AA, Zeng L, Takoudis CG, Hanada T, Bolis A, Bolino A, Chishti AH: Identification of erythrocyte p55/MPP1 as a binding partner of NF2 tumor suppressor protein/Merlin. Exp Biol Med (Maywood) 2009, 234: 255–262. doi:10.3181/0809-RM-275 10.3181/0809-RM-275View ArticleGoogle Scholar
- Stemmer-Rachamimov AO, Gonzalez-Agosti C, Xu L, Burwick JA, Beauchamp R, Pinney D, Louis DN, Ramesh V: Expression of NF2-encoded merlin and related ERM family proteins in the human central nervous system. J Neuropathol Exp Neurol 1997, 56: 735–742. 10.1097/00005072-199706000-00011View ArticlePubMedGoogle Scholar
- England JD, Asbury AK: Peripheral neuropathy. Lancet 2004, 363: 2151–2161. doi:10.1016/S0140–6736(04)16508–2 10.1016/S0140-6736(04)16508-2View ArticlePubMedGoogle Scholar
- Evans DG, Huson SM, Donnai D, Neary W, Blair V, Newton V, Harris R: A clinical study of type 2 neurofibromatosis. Q J Med 1992, 84: 603–618.PubMedGoogle Scholar
- Sperfeld AD, Hein C, Schroder JM, Ludolph AC, Hanemann CO: Occurrence and characterization of peripheral nerve involvement in neurofibromatosis type 2. Brain 2002, 125: 996–1004. 10.1093/brain/awf115View ArticlePubMedGoogle Scholar
- MacCollin M (1999) Clinical aspects. In: Friedman JM, Gutmann DH, MacCollin M, Riccardi VM (eds). Johns Hopkins University Press, Baltimore, pp 299–326
- Kimura J: Electrodiagnosis in Diseases of Nerve and Muscle: Principles and Practice. Oxford University Press, USA, City; 2001.Google Scholar
- Grazzi L, Chiapparini L, Parati EA, Giombini S, D'Amico D, Leone M, Bussone G: Type II neurofibromatosis presenting as quadriceps atrophy. Ital J Neurol Sci 1998, 19: 94–96. 10.1007/BF02427564View ArticlePubMedGoogle Scholar
- Hagel C, Lindenau M, Lamszus K, Kluwe L, Stavrou D, Mautner VF: Polyneuropathy in neurofibromatosis 2: clinical findings, molecular genetics and neuropathological alterations in sural nerve biopsy specimens. Acta Neuropathol 2002, 104: 179–187. doi:10.1007/s00401–002–0535–7 10.1007/s00401-002-0535-7View ArticlePubMedGoogle Scholar
- Baumer P, Mautner VF, Baumer T, Schuhmann MU, Tatagiba M, Heiland S, Kaestel T, Bendszus M, Pham M: Accumulation of non-compressive fascicular lesions underlies NF2 polyneuropathy. J Neurol 2013, 260: 38–46. doi:10.1007/s00415–012–6581–8 10.1007/s00415-012-6581-8View ArticlePubMedGoogle Scholar
- Iwata A, Kunimoto M, Inoue K: Schwann cell proliferation as the cause of peripheral neuropathy in neurofibromatosis-2. J Neurol Sci 1998, 156: 201–204. 10.1016/S0022-510X(98)00032-XView ArticlePubMedGoogle Scholar
- Iseki C, Takahashi Y, Wada M, Kawanami T, Kurita K, Kato T: A case of neurofibromatosis type 2 (NF2) presenting with late-onset axonal polyneuropathy. Rinsho Shinkeigaku 2009, 49: 419–423. 10.5692/clinicalneurol.49.419View ArticlePubMedGoogle Scholar
- Kuo HC, Chen SR, Jung SM, Wu Chou YH, Huang CC, Chuang WL, Wei KC, Ro LS (2010) Neurofibromatosis 2 with peripheral neuropathies: Electrophysiological, pathological and genetic studies of a Taiwanese family. Neuropathology doi:10.1111/j.1440–1789.2009.01099.x
- Bosch EP, Murphy MJ, Cancilla PA: Peripheral neurofibromatosis and peroneal muscular atrophy. Neurology 1981, 31: 1408–1414. 10.1212/WNL.31.11.1408View ArticlePubMedGoogle Scholar
- Overweg-Plandsoen WC, Brouwer-Mladin R, Merel P, de Vries L, Bijlsma EK: Neurofibromatosis type 2 in an adolescent boy with polyneuropathy and a mutation in the NF2 gene. J Neurol 1996, 243: 724–726. 10.1007/BF00873979View ArticlePubMedGoogle Scholar
- Perrot R, Berges R, Bocquet A, Eyer J: Review of the multiple aspects of neurofilament functions, and their possible contribution to neurodegeneration. Mol Neurobiol 2008, 38: 27–65. doi:10.1007/s12035–008–8033–0 10.1007/s12035-008-8033-0View ArticlePubMedGoogle Scholar
- Maeda M, Matsui T, Imamura M, Tsukita S: Expression level, subcellular distribution and rho-GDI binding affinity of merlin in comparison with Ezrin/Radixin/Moesin proteins. Oncogene 1999, 18: 4788–4797. doi:10.1038/sj.onc.1202871 10.1038/sj.onc.1202871View ArticlePubMedGoogle Scholar
- Bretscher A, Edwards K, Fehon RG: ERM proteins and merlin: integrators at the cell cortex. Nat Rev Mol Cell Biol 2002, 3: 586–599. doi:10.1038/nrm882 10.1038/nrm882View ArticlePubMedGoogle Scholar
- Corfas G, Velardez MO, Ko CP, Ratner N, Peles E: Mechanisms and roles of axon-Schwann cell interactions. J Neurosci 2004, 24: 9250–9260. doi:10.1523/JNEUROSCI.3649–04.2004 10.1523/JNEUROSCI.3649-04.2004View ArticlePubMedGoogle Scholar
- Hanemann CO: Magic but treatable? Tumours due to loss of merlin. Brain 2008, 131: 606–615. doi:10.1093/brain/awm249 10.1093/brain/awm249View ArticlePubMedGoogle Scholar
- Denisenko N, Cifuentes-Diaz C, Irinopoulou T, Carnaud M, Benoit E, Niwa-Kawakita M, Chareyre F, Giovannini M, Girault JA, Goutebroze L: Tumor suppressor schwannomin/merlin is critical for the organization of Schwann cell contacts in peripheral nerves. J Neurosci 2008, 28: 10472–10481. doi:10.1523/JNEUROSCI.2537–08.2008 10.1523/JNEUROSCI.2537-08.2008View ArticlePubMedGoogle Scholar
- Thaxton C, Bott M, Walker B, Sparrow NA, Lambert S, Fernandez-Valle C: Schwannomin/merlin promotes Schwann cell elongation and influences myelin segment length. Mol Cell Neurosci 2011, 47: 1–9. doi:10.1016/j.mcn.2010.12.006 10.1016/j.mcn.2010.12.006View ArticlePubMedGoogle Scholar
- Jessen KR, Mirsky R: The origin and development of glial cells in peripheral nerves. Nat Rev Neurosci 2005, 6: 671–682. doi:10.1038/nrn1746 10.1038/nrn1746View ArticlePubMedGoogle Scholar
- Michailov GV, Sereda MW, Brinkmann BG, Fischer TM, Haug B, Birchmeier C, Role L, Lai C, Schwab MH, Nave KA: Axonal neuregulin-1 regulates myelin sheath thickness. Science 2004, 304: 700–703. doi:10.1126/science.1095862 10.1126/science.1095862View ArticlePubMedGoogle Scholar
- Taveggia C, Zanazzi G, Petrylak A, Yano H, Rosenbluth J, Einheber S, Xu X, Esper RM, Loeb JA, Shrager P, Chao MV, Falls DL, Role L, Salzer JL: Neuregulin-1 type III determines the ensheathment fate of axons. Neuron 2005, 47: 681–694. doi:10.1016/j.neuron.2005.08.017 10.1016/j.neuron.2005.08.017View ArticlePubMedPubMed CentralGoogle Scholar
- Scherer SS, Salzer JL: Axon-Schwann cell interactions during peripheral nerve degeneration and regeneration. Glial Cell Dev: Basic Princ Clinl Relevance 2001, 2: 165–196.Google Scholar
- Gijtenbeek JM, Gabreels-Festen AA, Lammens M, Zwarts MJ, van Engelen BG: Mononeuropathy multiplex as the initial manifestation of neurofibromatosis type 2. Neurology 2001, 56: 1766–1768. 10.1212/WNL.56.12.1766View ArticlePubMedGoogle Scholar
- Fricker FR, Bennett DL: The role of neuregulin-1 in the response to nerve injury. Future Neurol 2011, 6: 809–822. 10.2217/fnl.11.45View ArticlePubMedPubMed CentralGoogle Scholar
- Denisenko-Nehrbass N, Goutebroze L, Galvez T, Bonnon C, Stankoff B, Ezan P, Giovannini M, Faivre-Sarrailh C, Girault JA: Association of Caspr/paranodin with tumour suppressor schwannomin/merlin and beta1 integrin in the central nervous system. J Neurochem 2003, 84: 209–221. 10.1046/j.1471-4159.2003.01503.xView ArticlePubMedGoogle Scholar
- Zhang C, Susuki K, Zollinger DR, Dupree JL, Rasband MN: Membrane domain organization of myelinated axons requires betaII spectrin. J Cell Biol 2013, 203: 437–443. doi:10.1083/jcb.201308116 10.1083/jcb.201308116View ArticlePubMedPubMed CentralGoogle Scholar
- Salzer JL, Brophy PJ, Peles E: Molecular domains of myelinated axons in the peripheral nervous system. Glia 2008, 56: 1532–1540. doi:10.1002/glia.20750 10.1002/glia.20750View ArticlePubMedGoogle Scholar
- Gronholm M, Vossebein L, Carlson CR, Kuja-Panula J, Teesalu T, Alfthan K, Vaheri A, Rauvala H, Herberg FW, Tasken K, Carpen O: Merlin links to the cAMP neuronal signaling pathway by anchoring the RIbeta subunit of protein kinase A. J Biol Chem 2003, 278: 41167–41172. doi:10.1074/jbc.M306149200 10.1074/jbc.M306149200View ArticlePubMedGoogle Scholar
- Yamauchi J, Miyamoto Y, Kusakawa S, Torii T, Mizutani R, Sanbe A, Nakajima H, Kiyokawa N, Tanoue A: Neurofibromatosis 2 tumor suppressor, the gene induced by valproic acid, mediates neurite outgrowth through interaction with paxillin. Exp Cell Res 2008, 314: 2279–2288. doi:10.1016/j.yexcr.2008.03.019 10.1016/j.yexcr.2008.03.019View ArticlePubMedGoogle Scholar
- Schulz A, Kyselyova A, Baader SL, Jung MJ, Zoch A, Mautner VF, Hagel C, Morrison H (2014) Neuronal merlin influences ERBB2 receptor expression on Schwann cells through neuregulin 1 type III signalling. Brain doi:10.1093/brain/awt327
- Citri A, Skaria KB, Yarden Y: The deaf and the dumb: the biology of ErbB-2 and ErbB-3. Exp Cell Res 2003, 284: 54–65. 10.1016/S0014-4827(02)00101-5View ArticlePubMedGoogle Scholar
- Zanazzi G, Einheber S, Westreich R, Hannocks MJ, Bedell-Hogan D, Marchionni MA, Salzer JL: Glial growth factor/neuregulin inhibits Schwann cell myelination and induces demyelination. J Cell Biol 2001, 152: 1289–1299. 10.1083/jcb.152.6.1289View ArticlePubMedPubMed CentralGoogle Scholar
- Massa R, Palumbo C, Cavallaro T, Panico MB, Bei R, Terracciano C, Rizzuto N, Bernardi G, Modesti A: Overexpression of ErbB2 and ErbB3 receptors in Schwann cells of patients with Charcot-Marie-tooth disease type 1A. Muscle Nerve 2006, 33: 342–349. doi:10.1002/mus.20460 10.1002/mus.20460View ArticlePubMedGoogle Scholar
- Sherman LS, Rizvi TA, Karyala S, Ratner N: CD44 enhances neuregulin signaling by Schwann cells. J Cell Biol 2000, 150: 1071–1084. 10.1083/jcb.150.5.1071View ArticlePubMedPubMed CentralGoogle Scholar
- Thaxton C, Lopera J, Bott M, Fernandez-Valle C: Neuregulin and laminin stimulate phosphorylation of the NF2 tumor suppressor in Schwann cells by distinct protein kinase A and p21-activated kinase-dependent pathways. Oncogene 2008, 27: 2705–2715. doi:10.1038/sj.onc.1210923 10.1038/sj.onc.1210923View ArticlePubMedGoogle Scholar
- Clark JJ, Provenzano M, Diggelmann HR, Xu N, Hansen SS, Hansen MR: The ErbB inhibitors trastuzumab and erlotinib inhibit growth of vestibular schwannoma xenografts in nude mice: a preliminary study. Otol Neurotol 2008, 29: 846–853. Doi 10.1097/MAO.0b013e31817f7398 10.1097/MAO.0b013e31817f7398View ArticlePubMedPubMed CentralGoogle Scholar
- Ammoun S, Cunliffe CH, Allen JC, Chiriboga L, Giancotti FG, Zagzag D, Hanemann CO, Karajannis MA: ErbB/HER receptor activation and preclinical efficacy of lapatinib in vestibular schwannoma. Neuro-Oncology 2010, 12: 834–843. doi:10.1093/neuonc/noq012 10.1093/neuonc/noq012View ArticlePubMedPubMed CentralGoogle Scholar
- Parrinello S, Noon LA, Harrisingh MC, Wingfield Digby P, Rosenberg LH, Cremona CA, Echave P, Flanagan AM, Parada LF, Lloyd AC: NF1 loss disrupts Schwann cell-axonal interactions: a novel role for semaphorin 4 F. Genes Dev 2008, 22: 3335–3348. doi:10.1101/gad.490608 10.1101/gad.490608View ArticlePubMedPubMed CentralGoogle Scholar
- Joseph NM, Mosher JT, Buchstaller J, Snider P, McKeever PE, Lim M, Conway SJ, Parada LF, Zhu Y, Morrison SJ: The loss of Nf1 transiently promotes self-renewal but not tumorigenesis by neural crest stem cells. Cancer Cell 2008, 13: 129–140. doi:10.1016/j.ccr.2008.01.003 10.1016/j.ccr.2008.01.003View ArticlePubMedPubMed CentralGoogle Scholar
- James MF, Manchanda N, Gonzalez-Agosti C, Hartwig JH, Ramesh V: The neurofibromatosis 2 protein product merlin selectively binds F-actin but not G-actin, and stabilizes the filaments through a lateral association. Biochem J 2001, 356: 377–386. 10.1042/0264-6021:3560377View ArticlePubMedPubMed CentralGoogle Scholar
- Xu HM, Gutmann DH: Merlin differentially associates with the microtubule and actin cytoskeleton. J Neurosci Res 1998, 51: 403–415. 10.1002/(SICI)1097-4547(19980201)51:3<403::AID-JNR13>3.0.CO;2-7View ArticlePubMedGoogle Scholar
- Hadfield KD, Smith MJ, Urquhart JE, Wallace AJ, Bowers NL, King AT, Rutherford SA, Trump D, Newman WG, Evans DG: Rates of loss of heterozygosity and mitotic recombination in NF2 schwannomas, sporadic vestibular schwannomas and schwannomatosis schwannomas. Oncogene 2010, 29: 6216–6221. doi:10.1038/onc.2010.363 10.1038/onc.2010.363View ArticlePubMedGoogle Scholar
- Stemmer-Rachamimov AO, Ino Y, Lim ZY, Jacoby LB, MacCollin M, Gusella JF, Ramesh V, Louis DN: Loss of the NF2 gene and merlin occur by the tumorlet stage of schwannoma development in neurofibromatosis 2. J Neuropathol Exp Neurol 1998, 57: 1164–1167. 10.1097/00005072-199812000-00008View ArticlePubMedGoogle Scholar
- Huang YY, Kandel ER, Varshavsky L, Brandon EP, Qi M, Idzerda RL, McKnight GS, Bourtchouladze R: A genetic test of the effects of mutations in PKA on mossy fiber LTP and its relation to spatial and contextual learning. Cell 1995, 83: 1211–1222. 10.1016/0092-8674(95)90146-9View ArticlePubMedGoogle Scholar
- Wassink TH, Brzustowicz LM, Bartlett CW, Szatmari P: The search for autism disease genes. Ment Retard Dev Disabil Res Rev 2004, 10: 272–283. doi:10.1002/mrdd.20041 10.1002/mrdd.20041View ArticlePubMedGoogle Scholar
- Penzes P, Cahill ME, Jones KA, VanLeeuwen JE, Woolfrey KM: Dendritic spine pathology in neuropsychiatric disorders. Nat Neurosci 2011, 14: 285–293. doi:10.1038/nn.2741 10.1038/nn.2741View ArticlePubMedPubMed CentralGoogle Scholar
- Murakoshi H, Wang H, Yasuda R: Local, persistent activation of Rho GTPases during plasticity of single dendritic spines. Nature 2011, 472: 100–104. doi:10.1038/nature09823 10.1038/nature09823View ArticlePubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.