Figure 3

Parallel identification of the same neurons in DL stained with KB and immunostaining. (a) Low-power view of the dorsolateral region of sacral spinal cord on KB, and (b) immunostaining of the rectangle area in (a). High-power view of this region on KB which enable us to manually trace neurons (c) and immunostaining (d). Rounded inclusion (thick arrow) and granular/dot-like deposits (thin arrow) shown in (d) which is a merge composed of DAPI (light blue) (e), phosphorylated TDP43 (green) (f), p62 (blue) (g) and non-phosphorylated TDP43 (red) (h). Rounded inclusion positive for non-phosphorylated TDP43, phosphorylated TDP43 and p62 (f, g, h). Granular deposits only positive for non-phosphorylated TDP43 (h). The same neuron, indicated by asterisk (*), harboring a rounded inclusion in (a-d, f-h). Bars = (a) 500 μm, (b) 200 μm, (c-h) 50 μm.