Figure 5
From: A central role for dityrosine crosslinking of Amyloid-β in Alzheimer’s disease
The stability of crosslinked fibrils. a) Oxidized and non-oxidized Aβ42 fibrils were examined using dityrosine fluorescence after prolonged incubation at −80°C showing a strong intensity signal at 420 nm for oxidized but not non-oxidized fibrils. b) Formic acid was used to dissolve the fibrils and the concentration of Aβ42 in solution was compared before and after formic acid dissolution for oxidized and non-oxidized fibrils. c) Electron micrographs of oxidized fibrils before and after formic acid treatment showing that the dityrosine crosslinked fibrils are resistant to formic acid. d) Electron micrographs of non-oxidized fibrils before and after formic acid treatment showing the fibrils are susceptible to damage by formic acid. Scale bars represent 0.2 μm.