Table 3 Antibodies and dilutions used
Antigens | Type | Clone / Name | Dilut. | Origin | Double stains with |
|---|---|---|---|---|---|
α-smooth muscle actin | mouse monoclonal | 1A4 | 1:500 | Sigma | HNE |
CD45 (leukocyte antigen) | mouse monoclonal | 2B11 + PD7/26 | 1:100 | DAKO | Oil-Red-O |
CD68 (macrophage) | mouse monoclonal | PG-M1 | 1:100 | DAKO | 15-LOX |
15-Lipoxygenase | rabbit polyclonal | CheY | 1:1000 | (18) | CD68 |
Native or oxidized LDL epitopes | |||||
ApoB-100 | mouse monoclonal | MB47 | 1:500 | (10) | - |
Minimally modified LDL | mouse monoclonal | Ox4E6 | 1:200-1000 | (13) | 1A4, YE |
Copper oxidized LDL | guinea-pig polyclonal | YE | 1:500 | (14) | Ox4E6 |
Hydroxynonenal (OxLDL) | guinea-pig polyclonal | HNE | 1:1000 | (11) | 1A4, MDA-2 |
Malondialdehyde (OxLDL) | mouse monoclonal | MDA-2 | 1:50 | (11) | EO6 |
Natural anti-OxLDL IgM from ApoE mice | mouse monoclonal | EO6 | 1:500-1000 | (12) | 1A4, MDA2 |