Table 1 The ingredients of qRT-QuIC preparation
Component | volume |
|---|---|
10 x QuIC buffer | 10 μl |
(100 mM NaH2PO4, 100 mM Na2HPO4, 10 mM EDTA, 1.3 M NaCl, pH 6.9) | |
dd H2O | 35 μl for standard curve, not for testing tissue extract |
3 M NaCl | 10 μl |
rPrPsen (0.3 mg/ml) | 34 μl (final 0.1 mg/ml) |
1 mM thioflavin T | 1 μl |
seed | 10 μl for standard curve, 45 μl for testing tissue extract |
total volume of each RT-QuIC | 100 μl |