Figure 5

Double-label immunofluorescence of MT1/2 and MT3 in a normal control DRG and a DRG of an FA patient. (a)-(c) normal control; (d)-(f) FA; (a) and (d) MT1/2 (Alexa488, green); (b) and (e) MT3 (Cy3, red); (c) and (f) merged images. MT1/2 immunoreactivity is restricted to normal (a) and hyperplastic satellite cells in FA (d). MT3 immunofluorescence is localized in the cytoplasm of a normal large DRG neuron (b) and in satellite cells, in which it co-localizes with MT1/2 (a-c, arrows). In FA (e), one of three small DRG neurons shows very little MT3 immunofluorescence (N) while two others remain strongly fluorescent. The thickened layers of satellite cells around the 3 neurons show relatively low MT3 fluorescence (e). Bars, 20 μm.