Fig. 5

Overlap of differentially expressed genes between melanoma patients and gene ontology (GO) enrichment analysis of top differentially expressed genes. A, Number of candidate genes that were shared across multiple patients. The x-axis shows the number of patients that commonly share the corresponding differentially expressed genes. The y-axis shows the total number of shared differentially expressed genes (shared genes with decreased (blue)/increased (red) expression in patient-matched intra- compared to extracranial metastasis pairs). B, GO analysis of biological processes of the 242 candidate genes with increased expression in intracranial metastases compared to their corresponding extracranial metastases that were shared across at least eight or more patients. Colors of the bars mark biological process groups (red: synaptic processes, orange: brain-specific cell development, dark brown: neuronal processes, light brown: no specific group). C, GO analysis of biological processes of the 459 candidate genes with decreased expression in intracranial metastases compared to their corresponding extracranial metastases that were shared across at least eight or more patients. Colors of the bars mark biological process groups (purple: immune response, green: activation of immune system, darkgreen: chemokine-related processes, blue: immune-cell development, light blue: other immune-related processes)